Jairus B. Bowne1, Tim A. Erwin1, Juan Juttner2, Thorsten Schnurbusch2, Peter Langridge2, Antony Bacic1,3,4,5, Ute Roessner1,5
1.Australian Centre for Plant Functional Genomics, School of Botany, The University of Melbourne, 3010 Victoria, Australia; 2.Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, 5064 SA, Australia; 3,Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Rd, The University of Melbourne, 3010 Victoria, Australia; 4.ARC Centre for Excellence for Plant Cell Walls, School of Botany, The University of Melbourne, 3010 Victoria, Australia; 5.Metabolomics Australia, School of Botany, The University of Melbourne, 3010 Victoria, Australia
Abstract: Drought has serious effects on the physiology of cereal crops. At the cellular and specifically the metabolite level, many individual compounds are increased to provide osmoprotective functions, prevent the dissociation of enzymes, and to decrease the number of reactive oxygen species present in the cell. We have used a targeted GC–MS approach to identify compounds that differ in three different cultivars of bread wheat characterized by different levels of tolerance to drought under drought stress (Kukri, intolerant; Excalibur and RAC875, tolerant). Levels of amino acids, most notably proline, tryptophan, and the branched chain amino acids leucine, isoleucine, and valine were increased under drought stress in all cultivars. In the two tolerant cultivars, a small decrease in a large number of organic acids was also evident. Excalibur, a cultivar genotypically related to Kukri, showed a pattern of response that was more similar to Kukri under well-watered conditions. Under drought stress, Excalibur and RAC875 had a similar response; however, Excalibur did not have the same magnitude of response as RAC875. Here, the results are discussed in the context of previous work in physiological and proteomic analyses of these cultivars under drought stress.
Key words: abiotic/environmental stress; metabolomics; drought; wheat
Source: Molecular Plant.2012 5(2)